Detention warrants were issued for 171 academics and former personnel of the now-defunct Fatih University on Wednesday.
State-run Anadolu news agency reported that warrants were issued for people who used to work for Fatih University and that police carried out operations in 29 provinces including Istanbul and rounded up 54 of the suspects.
Fatih University was earlier closed over ties to the Gulen movement which the government accuses of masterminding the July 15, 2016 coup attempt.
The movement denies involvement in the failed takeover, however nearly 130,000 people have been detained and 60,000 arrested over Gulen links so far.
Also, at least 3,008 universities, schools and dormitories were shuttered over similar charges.